Second , a prokaryotic expression construct , obtained from invitrogen transformed into prokaryotic and induced to express vp1 protein . the expressed vp1 fusion protein was purified by affinity chromatography using glutathione - agarose resin and used in elisa and western bolt analysis as the antigen . the elisa and western blot results showed that the anti - fmdv antibody was elicited specifically against vp1 antigen 第二，为了得到抗原蛋白，将vp1的原核表达质粒pgex - 4t - vp1转化入大肠杆菌bl21中，经iptg诱导，裂解细胞后用琼脂糖珠进行纯化，用elisa和westernblot进行检测，结果表明诱导表达出所需大小的融合蛋白。
After purification using glutathione sepharose 4b affinity chromatography and digestion with thrombin , the recombinant ntfs were found to be biologically active in the pc 12 cells neurite outgrowth assay . the assays demonstrated that the purified ntfs proteins exhibited normal activity , which is the first step in developing a comprehensive gene therapy for nerve diseases of the giant panda and the crested ibis 最后，对重组表达蛋白经glutathlonesepharose4bmicrospincolumn亲和纯化后，进行大鼠肾上腺嗜铬瘤细胞（ p2 ）神经营养因子的活性鉴定，发现其能够诱导神经细胞分化产生突触，即具有预期的生物学活性，表明所获表达蛋白为大熊猫nt
To provide theoretical basis for the enhancement of affinity chromatography , the synergy of multi fields , such as temperature field , chemical potential field and gravity field in affinity chromatography operated by a tapered bed with the continuous up - flow , was analyzed according to the phenomenological theory , and the relationships among the individual components within a field were investigated 摘要为了对亲和层析过程的强化提供理论依据，应用非平衡热力学唯象理论，分析了该过程中温度场、化学势场及重力场等各种场之间的协同关系，以及各自场内不同分量之间的协同关系。
After the gst fusion proteins were processed by factor xa and were purified by gluta - thione - sepharose 4b affinity chromatography , the recombinant protein hbrp with high purification would be acquired . during the process of separation and purification , the every result which can show the location of the aim protein was monitored by 10 % sds - page . the gst fusion proteins acquired finally were examined and analyzed by the methods of western - blottin 原核表达系统具有表达效率高，操作方便等特点，但缺乏翻译? 3 ?后修饰，以pgex载体表达的gst hbrp融合蛋白，具有表达的蛋白稳定性好，在体外易于纯化等优点，表达的gst融合蛋白可用gluthioneseph删sc4b进一步纯化一factorxa酶切与测分离，从而大量获得hbrp重组蛋白，而且一次回收率大约在90以上。
A method for preparation of antithrombin ( at - ) concentrated from human plasma ' s fraction has been described . after preliminary treatment of plasma with the cold ethanol , the isolation of at - iii from the precipitate was performed by affinity chromatography on heparin - sepharose cl - 6b ; desalted by dialysis and concentrated 从人体血浆中提取较高纯度的抗凝血酶( at - )的，通过低温乙醇处理血浆，得到的沉淀经肝素-琼脂糖两次亲和层析，并透析、浓缩获得at -的初品。
In order to detect the effect of human sperm mannose - ligand receptor on the fertilization ability , in the study reported here mannose - ligand receptors ( mrs ) were purified from human sperm by modified mannose - agarose gel affinity chromatography coloumn and determined protien concentration by lowry , preincubated zona - free hamster oocytes with four purified mannose - ligand receptor ( pmr ) concentrations before sperm penetration assay ( spa ) to test the pmrs cell biology nature of inhibition to fertilization 本研究用改良后的亲和层析法分离纯化mr ， lowry法测定其蛋白质浓度，在精子穿透试验( spermpenetrationassay ， spa )模型中定量研究其对精卵融合能力的影响并检测其细胞生物学活性；以已知浓度的pmr ( purifiedmannose - ligandreceptor )干预精子半透明带试验，观察用pmr预处理半透明带对精子与透明带结合的影响。
